General description

Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376.Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079.Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570.Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186.Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)